What is Phosphate Buffered Saline (PBS) used for?

Phosphate Buffered Saline (PBS) is commonly used in biological research and clinical laboratories as a buffer solution. It maintains a stable pH environment during experiments and processes like cell culture, washing cells, and diluting substances. Its isotonic nature helps to maintain cell integrity without causing lysis. PBS is especially invaluable in assays like ELISA or Western blotting, where consistent pH levels are imperative for reliable results.

What does '10x' mean in the context of PBS?

'10x' refers to the concentration of the PBS solution. It is a concentrated form that, when diluted, yields a 1x solution suitable for various biological applications. Researchers typically dilute the 10x PBS with distilled water to achieve the desired working concentration. This allows for flexibility in preparing the volume needed for experiments, making it a versatile choice for laboratories of all sizes.

Why is sterility important in PBS?

Sterility in PBS is crucial as it prevents contamination during biological experiments. Non-sterile solutions can harbor unwanted microorganisms, leading to skewed results and compromised cell viability. Using sterile PBS ensures the integrity of cell cultures, as well as the accuracy of assays. It’s particularly important in applications involving live cells, where any contamination can affect experimental outcomes and research credibility.

How should PBS be stored?

PBS should be stored at room temperature in a cool, dry place away from direct sunlight. If the PBS is opened, it’s advised to store it in a refrigerator to prolong its shelf life. Keeping it sealed and protected from contaminants is essential for maintaining its sterility and effectiveness in experiments. Proper storage practices contribute to consistent performance in laboratory settings.

Can PBS be used for protein or DNA handling?

Yes, PBS is often used in procedures involving proteins and DNA. Its buffer capacity helps maintain pH levels during extraction or purification processes, reducing the risk of degradation. Additionally, PBS is a common diluent for proteins in assays and is used to resuspend DNA for electrophoresis. This makes it an essential component in molecular biology and biochemistry applications.

What is the composition of PBS?

PBS typically contains sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. This specific composition ensures that the solution is isotonic and maintains a neutral pH. The buffering capacity of PBS is particularly helpful in various laboratory techniques, as it mimics the physiological conditions that cells experience. Understanding its composition can help in customizing solutions for unique experimental needs.

Is PBS safe to use with mammalian cells?

Yes, PBS is safe for use with mammalian cells. Its isotonic and buffered nature prevents osmotic shock and maintains cellular integrity. This makes it ideal for washing cells, diluting samples, and during procedures like trypsinization. Researchers frequently use it in conjunction with other reagents to ensure that cell viability is preserved throughout various handling processes.

How do I prepare a 1x PBS solution from 10x PBS?

To prepare a 1x PBS solution from 10x PBS, mix one part of the 10x solution with nine parts of distilled water. For example, if you want to prepare 100 mL of 1x PBS, combine 10 mL of 10x PBS with 90 mL of distilled water. This dilution will yield a working solution that can be utilized in various laboratory procedures, ensuring the proper buffer and pH for your experiments.

Can PBS be used for tissue culture applications?

Absolutely, PBS is widely used in tissue culture applications. Its balanced isotonic composition helps maintain cell health during washing and resuspension steps, ensuring that cells do not undergo osmotic stress. PBS is particularly useful when replacing culture media and can also be used for preparing cells for subsequent applications, such as transfections or cytotoxicity assays, supporting robust and reliable cell cultures.

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ABI

Phosphate Buffered Saline, PBS (10x), Sterile, 10 Bottles, 500mL Each

Our PBS formulation ensures pristine cell integrity without calcium and magnesium, ideal for your research needs.

ABI

Item #: 90793265

Phosphate Buffered Saline, PBS (10x), Sterile, 10 Bottles, 500mL Each

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You can buy Phosphate Buffered Saline, PBS 10x, Sterile, 10 Bottles, 500mL Each online through Ubuy. Ubuy offers a convenient platform for purchasing a variety of laboratory supplies. By shopping on Ubuy, you’ll find competitive options, ensuring that you can access the necessary reagents for your scientific needs swiftly and effectively.

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Item #: 90793265

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What Stands Out

Convenient Packaging

Each order includes ten 500mL bottles, optimizing convenience and reducing the need for frequent reordering, ideal for laboratories and research facilities with consistent buffer needs.

Sterile Formula

Sterilized for enhanced safety and reliability, ensuring contamination-free experiments, which is crucial for sensitive biological applications and high-accuracy research.

10x Concentration

This PBS formula offers a concentrated solution, allowing easy dilution for various applications, efficiently catering to diverse laboratory requirements without compromising quality.

Product Details

% Assay of Chemical(s)	10
Container Type	Bottle
Item Form	Liquid
Item Weight	13.8 pounds (6.26 kg)
Volume	500 ml
Brand Name	ABI
Manufacturer	American BioInnovations
Part Number	ABI-102-04
Brand	ABI
Item Volume	500 ml
Percentage assay	10
Package Information	Bottle

Who Should Buy?

Laboratory Researchers
Ideal for researchers needing a stable buffer for biological experiments involving cells or proteins.
Cell Culture
Essential for maintaining pH and osmotic balance while culturing cells in various lab applications.
Diagnostic Testing
Useful for laboratories conducting diagnostics that require a reliable saline solution as a reagent.

Field Use
Not suitable for outdoor experiments or field studies where conditions cannot be controlled.

Product Description

Phosphate Buffered Saline, PBS (10x), Sterile, 10 Bottles, 500mL Each

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Question: How to Shop Phosphate Buffered Saline, PBS (10x), Sterile, 10 Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffered Saline, PBS (10x), Sterile, 10 online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffered Saline, PBS (10x), Sterile, 10 Available to Shop Online in Saint Vincent?

Answer: Yes, at Ubuy Saint Vincent this product is available for you to shop at a reasonable price. The Phosphate Buffered Saline, PBS (10x), Sterile, 10 is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

Physiological buffer solution essential for cell culture.
Prevents cell aggregation with no calcium or magnesium.
Ideal for washing, transporting, and diluting cell samples.
Perfect for protein microarray hybridization and Western-Blot.
Sterile formulation ensures contamination-free experiments.
Versatile use in preparing reagents for various applications.

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Phosphate Buffered Saline, PBS (10x), Sterile, 10 Bottles, 500mL Each

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